anti-il-1r (rabbit polyclonal igg 1∶500)  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: Sequence of specific primers.

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: A shows TNF-α (30 kDa) and TNF-R 1 (55 kDa), IL-1β (17 kDa), IL-1R 1 (80 kDa) and β-actin (42 kDa) immunoreactive bands, respectively. Bar graphs in B, C show significant increase in the optical density of TNF-α and IL-1β, TNF-R 1 , IL-1R 1 following hypoxic exposure when compared with the corresponding controls (* P <0.01). Panels D and E show the graphical representation of the fold changes in TNF-α and IL-1β, TNF-R 1 , IL-1R 1 mRNA, respectively as quantified by normalization to the β-actin as an internal control. Significant increase in TNF-α and IL-1β, TNF-R 1 , IL-1R 1 mRNA levels in the PWM after the hypoxic exposure is evident when compared with controls (* P <0.01). The panels F show TNF-α (30 kDa) and IL-1β (17 kDa) immunoreactive protein bands in cultured control astrocytes and at 3 h after hypoxic exposure. G is bar graph showing changes in the optical density of TNF-α and IL-1β, respectively, following hypoxic exposure. H (TNF-α and IL-1β mRNA) show the graphical representation of the fold changes quantified by normalization to the β-actin as an internal control. Significant differences in protein and mRNA levels in astrocytes after the hypoxic exposure are evident when compared with controls (* P <0.01).

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: The co-localized expression of APC with TNF-R 1 and IL-1R 1 is depicted in C and F, I and L, respectively. Note the expression of TNF-R 1 and IL-1R 1 is upregulated after the hypoxic exposure. Scale bars: A–L, 50 µm.

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: Confocal images showing TNF-R 1 and IL-1R 1 expression (B, E, H, K, red) in primary cultured oligodendrocytes labeled with APC (A, D, G, J, green) in both control and hypoxia for 3 h. Note TNF-R 1 and IL-1R 1 immunofluroscence intensity is markedly enhanced after hypoxic exposure (E, K) in comparison with the control (B, H). Scale bars: A–L, 50 µm.

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Expressing, Cell Culture, Labeling

Journal: The FASEB Journal

Article Title: Bidirectional crosstalk between neutrophils and adipocytes promotes adipose tissue inflammation

doi: 10.1096/fj.201900477rr

Figure Lengend Snippet: Figure 3. Lipolysis induces neutrophil infiltration into the eWAT via an inflammasome and IL-1R–MyD88 pathway. A) WT mice were injected with PBS or CL316,243 (1 mg/kg) intraperitoneally (n = 13 per group). After 18 h, SVF was isolated from the eWAT, and cell number of SVF cells was calculated. B) Representative fluorescence-activated cell sorting (FACS) data (left) and cell numbers (right) of neutrophils (CD11b+/Ly6G+ cells) and macrophages (CD11b+/Ly6G2 cells) in SVF from WT mice injected with PBS or CL316,243 (1 mg/kg) (n = 13/group). C) eWAT from WT mice treated with PBS or CL316,243 (1 mg/kg) for 18 h was stained for MPO (red) and Mac-2 (green). The nuclei were counterstained and shown in blue. Scale bar, 25 mm. D) The indicated mice were injected with PBS or CL316,243 (1 mg/kg, i.p.) (n = 5–12/group). After 18 h, SVF was isolated from the eWAT, and cell numbers of neutrophils were calculated. E) WT mice were injected with Ct. IgG or anti-IL-1R (500 mg/mouse) twice (24 h between each injection) (n = 6/group). At 10 min after the second injection, mice were injected with CL316,243 (1 mg/kg). Representative FACS data are depicted (left). Cell numbers of total SVF cells, neutrophils, and macrophages were calculated after 18 h (right). Casp1, caspase-1; Ct., C. trachomatis; N.S., not significant. Data are shown as means 6 SE. All data are representative of $3 independent experiments. *P , 0.05, #P , 0.01, §P , 0.001.

Article Snippet: Purified anti-mouse IL-1b antibody, anti-mouse Gr-1 antibody, and anti-mouse IL-1R antibody were purchased from Bio X Cell (West Lebanon, NH, USA).

Techniques: Injection, Isolation, FACS, Staining